Figure 6 | Scientific Reports

Figure 6

From: Early life exposure to ethinylestradiol enhances subsequent responses to environmental estrogens measured in a novel transgenic zebrafish

Figure 6

Exposure Protocol Outline. ERE-Kaede-Casper embryos were initially separated into 48 h control (C) and EE2 (10 ng/L) initial-exposure (E) groups. After a subsequent 24 h non-exposure period, larvae were imaged and Kaede expression underwent photoconvertion (green to red fluorescence, 3 dpf). Various intervals of non-exposure were then adopted before a second estrogen exposure was conducted. Larvae from the two initial treatments (C and E) were each divided into two groups; one control exposure (C-Water and E-Water) and the second an estrogenic chemical exposure (C-Chemical and E-Chemical). Imaging was carried out at the final time point with subsequent image analysis for quantification of Kaede expression. The expression of the three nuclear ESR subtypes was also quantified at the final time point using qPCR.

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