Figure 1 | Scientific Reports

Figure 1

From: IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro

Figure 1

Down-regulation of IRE1α in the sciatic nerve after intrathecal injection of siRNA. (A) IRE1α was effectively reduced as measured by immunofluorescence. IRE1α was detected with an antibody against IRE1α, followed by a fluorescent-labeled secondary antibody (green). Data are expressed as mean ± SEM as the percentage of the respective controls and analyzed using one-way ANOVA with LSD analysis or unpaired Student’s t-test. (B) Representative Western blots using tissue extracts from the sciatic nerve and probed with antibodies against IRE1α. β-actin was used as loading control. Data are expressed as mean ± SEM indicated as the percentage of the respective controls and analyzed using Tamhane’s T2 analysis or unpaired Student’s t-test. ΔP < 0.05, ΔΔP < 0.01, compared to control; ##P < 0.01, compared to DPN; *P < 0.05 (N = 6 per group).

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