Figure 5 | Scientific Reports

Figure 5

From: IRE1α siRNA relieves endoplasmic reticulum stress-induced apoptosis and alleviates diabetic peripheral neuropathy in vivo and in vitro

Figure 5

High glucose-induced apoptosis and Ca2+ levels in RSC96 cells after IRE1α siRNA transfection. (A) FACS apoptosis assay results using FITC-Annexin V and PI staining at 24 h and 48 h. (B) Intracellular Ca2+ production measured with FACS analysis using Fluo-3 AM dye at 24 h and 48 h. (C) Apoptosis levels indicated as the percentage of the respective 25 mM glucose-treated cells. Data are expressed as mean ± SEM and analyzed using one-way ANOVA with LSD analysis. (D) Intracellular Ca2+ levels indicated as the percentage of the respective 25 mM glucose-treated cells. Data are expressed as mean ± SEM and analyzed using one-way ANOVA with LSD analysis. ΔP < 0.05, ΔΔP < 0.01, compared to25 mM glucose; ##P < 0.01, compared to 150 mM glucose; ★P < 0.05, ★★P < 0.01, Student’s unpaired t-test compared to 24 hours’ time point. *P < 0.05, **P < 0.01 (n = 4 per group).

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