Figure 7 | Scientific Reports

Figure 7

From: SJP-L-5 inhibits HIV-1 polypurine tract primed plus-strand DNA elongation, indicating viral DNA synthesis initiation at multiple sites under drug pressure

Figure 7

A Model of how SJP-L-5 inhibits PPT-primed HIV-1 plus-DNA synthesis. HIV-1 plus-strand DNA synthesis is initiated by cPPT or PPT in the central genome or 3′ end, leading to formation of a triple-strand flap in the center. Later, the viral DNA genome enters the nucleus and is integrated into the host DNA genome. In response to SJP-L-5 treatment, both cPPT and PPT primed plus-strand DNA synthesis are blocked, leading to a cPPT gap and a PPT gap in the center and the 3′ end of HIV-1 DNA genome. Under the pressure of this compound, HIV-1 uses multiple sites to initiate its plus-strand DNA synthesis within down-stream plus-strand DNA, forming five segmented plus-strand DNA bands and two PPT gaps (cPPT and PPT). Due to a lack of matured DNA (a triple-DNA flap), the impeded viral DNA fails to enter the host cell nucleus and cannot integrate into the host DNA genome.

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