Figure 5
Efflux assay of ELKs. (a) Cholesterol efflux from ABCA1-BHK cells as a function of peptide concentration. (b) Cholesterol efflux from ABCA1 transporter induced in macrophages as a function of peptide concentration. Results are expressed as the mean ± 1 S.D. of triplicates in (a) and (b). A positive control using 0.5 µM apoA-I protein showed 30% (a) and 26% (b) efflux after 18 h. (c) Human plasma incubation of ELKs causes HDL remodeling. (d) Cholesterol efflux by ABCA1 transporter-expressing cells treated with human pooled plasma pre-treated with ELKs. A previously described apoA-I mimetic peptide, 5A, was included as a positive control (c) and (d). Statistical analysis was performed comparing each peptide with control sample (plasma alone) using paired t test and presented as p < 0.05 = *.
