Figure 2

IgG1-immunoreactive proteins from Cryptococcus neoformans were detected with sera from representative infected but not naïve wild-type and IL-12-deficient mice. Whole cell proteins of C. neoformans strain 1841 separated by 2D electrophoresis were transferred to nitrocellulose membranes, which were thereafter incubated with sera from infected and naïve wild-type and gene-deficient mice diluted 1:1,000. IgG1-immunoreactive proteins were detected using sera from an infected wild-type (a), a naïve wild-type (b), an infected IL-12-deficient (c), and a naïve IL-12-deficient (d) mouse. Protein abundance as shown in the Coomassie staining did not correlate with the strength of the immunoreactive signal (Fig. 4). Only the spots that could be mapped on Coomassie-stained gels were numbered in the blot images. Bold numbers indicate strictly IgG1-reactive proteins while italic numbers mark proteins reactive with both, IgG1 and IgG2a antibodies. Images were cropped to improve clarity. Full-length blots without numbered protein spots are presented in Supplementary Fig. 2. Abbreviation: MM = molecular mass.