Table 1 Summary of steady-state experimental results.

From: Skeletal myosin binding protein-C isoforms regulate thin filament activity in a Ca2+-dependent manner

Experimental Assay

System

Concentration of N’ fragment

Results at lower Ca2+ (pCa 9–7)

Results at higher Ca2+ (pCa 6–4.5)

Force-ATPase

Adult Rat Papillary Muscle Fiber Bundles

10 µM

N/A

C0C2 and fsC1C2 increased force, Ca2+ sensitivity, ktr

In vitro Motility Assays

Mouse myosin and Native Thin Filaments

0.25 µM 0.10–2.0 µM

C0C2 and ssC1C2 activate NTF motility

C0C2 and fsC1C2 inhibit NTF motility

3D reconstruction of EM images

Reconstituted thin filament

6 µM

Graded shifts in tropomyosin position (C0C2 > ssC1C2 > fsC1C2)

N/A

  1. The table summarizes all steady-state experiments performed in the present studies, experimental systems and concentration of the MyBP-C fragments used. Results are summarized at lower and higher Ca2+ concentration.
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