Figure 1
From: RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment
Effect of shikonin on the cell viability in breast cancer cell lines. Different breast cancer cells. (A) MCF-7, (B) SK-BR-3, (C) MDA-MB231, and (D) human mammary epithelial cells, M10, were incubated with different concentrations of shikonin (0–50 μM) for 24 h. The cell viability was determined by the MTT assay. Data points represent the mean ± SD of three independent experiments. IC50 values were calculated by GraphPad Prism 4.0 software using a sigmoidal curve fit based on nonlinear regression. Statistical significance was assessed by one-way ANOVA followed by Tukey post-hoc test and represented as follows: *P < 0.05 and **P < 0.01 vs. shikonin 0 μM (DMSO control).
