Figure 4
Characterization of 3D spheroids, generated from SBA iPSC-derived neural rosettes. (A) Diameter size (μm) of CTRL iPSC and SBA neural rosettes-derived 3D spheroids shown as percentage. Data are plotted as average ± SEM. **P < 0.01 CTRL iPSC vs SBA and SBA vs SBA + FA, *P < 0.05 CTRL iPSC vs CTRL iPSC + FA. N = 4 independent experiment per iPSC/hESC line. (B) Enumeration of Ki67+ cells in CTRL iPSC and SBA neural rosettes-derived 3D spheroids shown as percentage. NS, not significant. Data are plotted as average ± SEM ****P < 0.0001 CTRL iPSC vs SBA, SBA vs SBA + FA, ***P < 0.001 CTRL iPSC vs CTRL iPSC + FA. N = 4 independent experiment per iPSC line. (C) IF staining for tight junction protein marker ZO-1 (green) and Ki67 (red) in CTRL iPSC and SBA-derived 3D spheroids sections. Representative images of CTRL iPSC1#2 and SBA2#1 are shown. Nuclei were counterstained with DAPI (blue). Scale bar = 200 μm for ZO-1, 100 μm for Ki67. (D) IF staining for early neural differentiation marker β–III Tubulin (red) in CTRL iPSC SBA neural rosettes-derived 3D spheroids sections. Nuclei were counterstained with DAPI (blue). Representative images of CTRL iPSC1#2 and SBA2#1 are shown. Scale bar = 200 μm. (E) Enumeration of β–III Tubulin+ cells in CTRL iPSC and SBA neural rosettes-derived spheroids shown as percentage. Data are plotted as average ± SEM. *P < 0,05 CTRL vs CTRL + FA, **P < 0.01 CTRL iPSC vs SBA, CTRL iPSC + FA vs SBA + FA, SBA vs SBA + FA ****P < 0.0001 CTRL iPSC + FA vs SBA. N = 4 independent experiment per line. (F) IF staining for PAX6 (green), NESTIN (red) and β–III Tubulin (blue) in SBA neural rosettes-derived 3D spheroids sections. Representative images of SBA2#1 are shown. Scale bar = 200 μm.
