Figure 5
From: A novel flatworm-specific gene implicated in reproduction in Macrostomum lignano
Expression pattern of Mlig-sperm1 gene. (a) Whole mount in situ hybridization demonstrating the specific expression of the Mlig-sperm1 gene in the testes of an adult worm (blue precipitation). (b) Fluorescent in situ hybridization of the Mlig-sperm1 gene (red) in an adult worm, combined with a DAPI labeling of all nuclei in the worm (blue). This confirms the testes-specific expression of Mlig-sperm1. (c–f) Triple labeling including Mlig-sperm1 FISH (c), DAPI labeling (d), mitotic labeling with the anti-phospho-histone H3 antibody (e), and the overlay of all three (f). The Mlig-sperm1 (red) clearly encircles DAPI-labeled nuclei (blue) indicating the expression within testicular cells. Importantly, this includes the nuclei of the mitotic cells (green), demonstrating expression in the proliferating testicular cells. Scale bars: 100 µm (a,b), and 25 µm (c–f).
