Figure 3 | Scientific Reports

Figure 3

From: WDR1 is a novel EYA3 substrate and its dephosphorylation induces modifications of the cellular actin cytoskeleton

Figure 3

EYA3 Y508F and EYA3 Y532F proteins are new, specific trapping mutants for EYA3 phosphatase. Src kinase was co-expressed in 293 T cells with: (a) myc-tagged EYA3 single point mutants (D311N, Y508F and Y532F) and VSV-G-tagged EYA3 D309N. The images are cropped from original blots. (b) VSV-G-EYA3 (WT and D309N) and myc-tagged EYA3 (WT, D309N, Y508F and D309N Y508F). EYA proteins were immunoprecipitated from cell lysates and trapped proteins were analyzed by western blotting with indicated antibodies. WCLs were immunoblotted with anti-Src and anti-actin. Full-length blots are presented in Supplementary Fig. S18.

Back to article page