Figure 1
From: ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner
Overexpression of ZFP36L1 and ZFP36L2 decreased the viability of T-REx-293 cells. T-REx-293/pcDNA5-TO (control), T-REx-293/HA-ZFP36L1 (HA-L1), and T-REx-293/HA-ZFP36L2 (HA-L2) cells were treated with 1 μg/mL of doxycycline (Dox) to induce ZFP36L1 or ZFP36L2 expression for 1, 2, and 3 days. (a) Total proteins were collected, and the protein expressions of hemagglutinin (HA)-tagged ZFP36L1 or HA-tagged ZFP36L2 were examined using western blot analysis with anti-ZFP36L1, anti-ZFP36L2, or anti-HA antibodies. (b) Cell viability was analyzed using an MTT method. Values were normalized to control cells without Dox at day 1 (lane 1). (c) The numbers of viable cells were determined by counting the trypan blue-excluding cells in a hemocytometer. (d) Cytotoxicity was analyzed using a lactate dehydrogenase cytotoxicity detection kit. (e) After 72 h of treatment, the cells were photographed; the representative photographs are shown. Each value is presented as the mean ± SD of three independent experiments. *p < 0.05, compared with control cells.
