Figure 5
From: ZFP36L1 and ZFP36L2 inhibit cell proliferation in a cyclin D-dependent and p53-independent manner
Effects of the overexpression of ZFP36L1 and ZFP36L2 on expressions of cell-cycle-related proteins in human colorectal cancer cells. HCT116 p53+/+, HCT116 p53−/−, and SW620 cells were transduced with a lentiviral expression vector (at a multiplicity of infection of 3) to express enhanced green fluorescent protein (eGFP; control), hemagglutinin (HA)-tagged ZFP36L1 (HA-L1), or HA-tagged ZFP36L2 (HA-L2) protein for 1, 2, and 3 days. Total proteins were collected, and expressions of cell-cycle-related proteins were detected using western blot analysis.
