Figure 2

(a) RAGE protein expression in the kidney of Cont and DOCA mice (n = 3, respectively), (b) RAGE protein expression in lung of WT and RAGE-KO mice, (c) SBP levels of Cont, DOCA, and DOCA–RAGE-KO mice (n = 5–8 per group), ^§p < 0.05 vs Cont, (d) UAE levels of Cont, DOCA, and DOCA–RAGE-KO mice (n = 5–6 per group), (e and f) mesangial matrix expansion and tubulointerstitial fibrosis assessed by periodic acid-Schiff and Masson’s trichrome stains, respectively, in Cont, DOCA, and DOCA–RAGE-KO mice (n = 5 per group), (g–i) immunohistochemical analysis for glomerular 8-OHdG, GTP-bound Rac1, and MR levels in Cont, DOCA, and DOCA–RAGE KO mice (n = 5 per group), (j) glomerular CML staining by immunohistochemistry (n = 4–5 per group), (k) podocin expression (n = 4–5 per group), (l) cortical mRNA expression levels of MR, SGK1, and TGF-β in Cont, DOCA, and DOCA–RAGE-KO mice (n = 5 per group). Data are presented as the mean ± SEM. ^#p < 0.05, ^##p < 0.01 vs. DOCA mice. All sections were 4-μm thin. Bars = 20 μm. RAGE, receptor for advanced glycation end products; Cont, control; WT, wild type; KO, knockout; 8-OHdG, 8-hydroxy-2ʹ-deoxyguanosine; MR, mineralocorticoid receptor; SGK1, serum/glucocorticoid regulated kinase1; TGF-β, transforming growth factor-β.