Figure 6

Effects of NOC-18-induced Rela (NF-kB p65) on odontogenic capacity of rDPSCs. (a,b) Expression of Rela (a) and tumor necrosis factor alpha (Tnf) (b) genes by real-time RT-PCR at the early (day 15) stages of the odontogenic differentiation. (c) Effect of NF-κB inhibitor PDCT (100 μM) on Tnf expression in rDPSCs at the early (day 15) stages of the odontogenic differentiation by real-time RT-PCR. (d–h) Effect of NF-κB inhibitor PDCT (100 μM) on the odontogenic differentiation of rDPSCs by real-time RT-PCR. Measurement of calcium contents by Alizarin Red-S staining (d). Expression of Runx2 (e) and Alp (f) at the early (day 15) stage and Bglap (g) and Dspp (h) at the late (day 25) stage. (a–h) n = 3 per group; *p < 0.05. Graph bars are the means ± SEM. (a–c,e–h) Each gene was normalized to 18S ribosomal RNA (18S) in each sample.