Figure 5
From: Novel ubiquitin-independent nucleolar c-Myc degradation pathway mediated by antizyme 2
AZ2 contributes to downregulation of c-Myc under hypoxic and glucose free conditions. (a) Panc-1 ells were cultured under normal or hypoxic condition in medium with or without glucose. The cells were harvested at indicated times and endogenous c-Myc levels were analyzed by immunoblotting using anti c-Myc antibody. β-Actin bands are also shown. Nor, normoxia, Hyp, hypoxia. (b) Statistical analysis of (a). (c) Panc-1 cells were transfected with control, AZ1 or AZ2 siRNA (20 nM). After 24 h, the medium was changed to glucose-free medium and cells were cultured 48 h under hypoxic condition. Endogenous c-Myc levels were analyzed as in (a). Nor-Glu, normoxia and glucose-free, Hyp-Glu, hypoxia and glucose-free, Hyp + Glu, hypoxia in the presence of glucose, Data in a-c represent the mean ± SD calculated from three independent experiments.
