Figure 3
From: Characterization of viral RNA splicing using whole-transcriptome datasets from host species
Splicing profiles of AdDNV and PcDNV. (A) Detected splicing junctions of AdDNV_1KITE. (B) Detected splicing junction models of PcDNV_1KITE: Solid gray areas represented the TopHat2 mapping profiles and each color-coded block represented a splicing junction. Red and purple blocks were forward and reverse junctions, respectively. The edge of each block represented the coverage of supporting reads and the length of a block represented the location of a splicing event. The number near each block was the coverage of supporting reads. The middle bridge showed the intron region from the splicing event. The block thickness represented frequency (the number of supporting reads) of the intron. Splice site compositions for donor sites, branch sites and acceptor sites of all GT-AG type introns in AdDNV_1KITE (Panel C) and PcDNV_1KITE (Panel D) were displayed using WebLogo. The overall height of each stack indicated the sequence conservation at that position, measured in bits. Proteins mediating the GT-AG splicing were labelled as snRNP (small nuclear ribonucleoproteins) and SR (splicing regulatory proteins). (E) Log2 scale of reads density of introns in the genome alignment: The Y-axis showed the expression levels (the number of reads) of intron related splicing events. Introns with forward junctions (red labels, at NS region) and reverse junctions (blue labels, at VP region) of PcDNV_1KITE (top half) and AdDNV_1KITE (bottom half) were shown in the genome alignment. Multiple splicing events (orange labels) were also displayed.
