Figure 3
Decreased gene and protein expression of Tnmd in Scx-deficient mice. (a–o) In situ hybridisation with antisense probes for Col1a1 (a,d,g,j,m), Scx (b,c), and Tnmd (e,h,k,n) or a sense probe for Tnmd (f,i,l,o) was performed on semiserial sections prepared from the legs of a Scx+/− embryo at E18.5 (a,b), a Scx+/− neonate at P1 (c–f,j–l) or a Scx−/− neonate at P1 (g–i,m–o). Sagittal sections of the knee joint (a,b,d–i) and the ankle (c,j–o) are shown. Black and hollowed arrowheads in (g,h,m, and n) indicate the tendons/ligaments that were positive or negative staining with the Tnmd antisense probe, respectively. (p–s) Double immunostaining of Chmd (green) and Col1 (red) (p,r) or Tnmd (red) (q,s) was performed on frozen sections prepared from Scx+/− (p,q) and Scx−/− (r,s) at P1. Sagittal sections of the ankle are shown. White and hollowed arrowheads in (r,s) indicate the regions of Achilles tendon that were positive and negative for staining with the Tnmd antibody, respectively. acl, anterior cruciate ligament; At, Achilles tendon; ca, calcaneus; fe, femur; pa, patella; pcl, posterior cruciate ligament; pl, patella ligament; qft, quadriceps femoris tendon. Scale bars, 500 μm.
