Figure 3
Reduced retinal flow volume and Müller cell gliosis in Akimba mouse. Laser speckle flowgraphy (LSFG) heat map of retinal blood flow in the animal models (A–D). Red indicates faster blood flow while blue indicates slower blood flow. Numbers 1–4 denotes four quadrants of the retina used for the analysis. Assessment of relative flow volume in the all four quadrants depicted significant attenuation of blood flow in Akimba (D, E). No differences were seen between the Kimba and Akita mice (E). Measurement of vessel diameter showed no difference in caliber amongst all four groups (F). Data represent mean \({\rm{\pm }}\) SD. n = 6 animals in each group. ***p < 0.001 compared to the WT. Akimba mouse showed maximal GFAP (J) stained cells compared with WT (G) or the parental strains (H, I). Similarly, GS immunostaining was observed throughout the entire inner retina and few cells migrating to the outer retina in the Akimba mice (N). Kimba retina showed similar staining patter but less intense (L) followed by Akita, where we found staining restricted to the inner retina (M) compared to the WT (K). n = 4 retina per group for immunohistochemistry, repeated twice. Arrowheads indicate positively stained cells. Scale bar, 50 µm.
