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Figure 2

From: Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Figure 2

Actin remodeling and growth cone collapse are early responses to Wallerian degeneration. (a) Low magnification micrograph of a DRG explant (Exp.) with a transection (segmented line) to induce Wallerian degeneration (right side of the transection). (b,c) Representative images (b) and quantification (c) of the time course of axonal fragmentation during Wallerian degeneration (WD) induced by axon transection. Scale bar: 100 μm. (d) Representative images of axon tips stained for alpha-tubulin (green) and F-actin (phalloidin, red) in control and transected explants. Scale bar: 2 μm. (e) Quantification of the proportion of growth cones in intact explants and 3 hour after axonal transection. (f,g) Representative images (f) and quantification (g) of the intensity of phalloidin staining in axonal shafts in intact and transected explants. Scale bar: 50 μm. Data are represented as mean ± SEM.

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