Figure 4 | Scientific Reports

Figure 4

From: Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration

Figure 4

Actin/Spectrin-MPS in DRG explants. (a,b) STED images of phalloidin (a) and βII-spectrin (b) stained axons, including their confocal image (top right). Arrowheads in the inserts delimit the line used to trace the intensity profiles shown in (c) and (d) respectively. Scale bars: 10 μm (STED), 20 μm (confocal) and 1 μm (inserts). (c,d) Intensity profiles from inserts in panels a and b, evidencing the periodic distribution of phalloiding (c) and βII-spectrin (d) staining and its characteristic mean period of ~190 nm. (e) Representative images of axonal segments containing longitudinal actin fibers (actin trails). Scale bar: 1 μm. (f) The distribution of actin-MPS was independent from the presence of actin bundles in the same 1 μm-segment, since there is a similar abundance of actin-MPS in segments without actin bundles or segmente with actin bundles. (g) Abundance of actin trails were similar in axons from hippocampal primary neurons (7DIV) and DRG sensory neurons (3DIV). (h) Percentage of segments showing a periodic distribution of actin or βII-spectrin in DRG axons grown for 3 days in vitro. (i) Spectrin-MPS abundance of cultures at different days in vitro. (j) Correlation coefficients of segments with spectrin-MPS from cultures at different days in vitro. Data are represented as mean ± SEM.

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