Figure 5

Vestibular organs are transduced following the RWM + CF injection. (a–c) Representative images of whole mounts of the CA of the PSCC, saccule, utricle and CAs of the LSCC and ASCC as indicated. Tissue was harvested 2 weeks after the RWM + CF injection (at P15–16), stained with Alexa Fluor 568-phalloidin (red) for labelling filamentous actin, and imaged for native eGFP (green). High magnification views of the regions marked with white dotted squares in the PSCC are shown separately and stained with Myo7a (gray) for labelling hair cells and imaged for native eGFP (green).