Figure 2 | Scientific Reports

Figure 2

From: Characterization of Retinal Ganglion Cell and Optic Nerve Phenotypes Caused by Sustained Intracranial Pressure Elevation in Mice

Figure 2

ICP elevation causes degeneration and loss of RGC axons and somas. (a) Axon counts for ICP (red) and Sham (black) groups after 2 weeks of ICP elevation. The number of axons per optic nerve is reduced after 2 weeks of ICP elevation (t test; p = 0.0097). N = 6 eyes for Sham and N = 5 eyes for ICP. Error bar = one SEM. (b–d). Representative optic nerve TEM images from Sham (b), moderate axon loss (c), and severe axon loss (d) after 2 weeks of ICP elevation. Note the relatively uniformly stained, small diameter axons in the Sham group (b) and different degrees of axon loss in (c) and (d). The upper inset indicates vacuolization inside the cytosol of an axon and lower inset indicates degeneration of an axolemma. Scale bar = 4 μm. 3000X magnification. (e–j). Representative confocal images from whole mount retinas stained for the RGC-specific markers Tuj1 (e/f,h/i) and RBPMS (f/g,i/j) after 2 weeks of either Sham or ICP elevation. Both RGC markers are reduced (Table 1). Scale bar = 20 μm. 40× magnification.

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