Figure 3

(a) Illustration of “two-site” binding in the model complex of ^hC5a-C5aR inserted into the POPC bilayer. (b) Interaction map of ^hC5a illustrating the “hot-spot” residues, respectively at the “site1” and “site2” of C5aR. “Hot-spot” residues that are known to modulate both binding and signaling upon mutation are highlighted in red.