Figure 5
From: Structure of prothrombin in the closed form reveals new details on the mechanism of activation
Kinetics of activation of open and closed conformations of prothrombin by the prothrombinase complex. (a) Graphical representation of the equilibrium between closed and open conformations of prothrombin that is shifted toward the closed form (>80%) under physiological conditions. ProTCC irreversibly locks prothrombin in the closed form. The mutation Y93A shifts the equilibrium toward the open form. (b,c) Activation of closed and open conformations of prothrombin by the prothrombinase complex monitored by chromogenic assay (b) or SDS-PAGE (c). Proteolytic fragments are indicated: prothrombin (proT), fragment-1.2.A (F1.2.A), fragment-1.2 (F1.2), prethrombin-2 (P2), B-chain (Bc), A-chain (Ac). Full-length gels are presented in Supplementary Fig. 5. Each measurement in panel b is the average of three individual determinations.
