Figure 6
From: Structure of prothrombin in the closed form reveals new details on the mechanism of activation
Structural architecture of meizothrombin in solution revealed by smFRET. (a) Cleavage of prothrombin (proT) at Arg320 generates meizothrombin (Mz). Fluorescent dyes were incorporated at positions S478 and S120, across the flexible Lnk2. (b) One-dimensional smFRET efficiency histogram for proT120/478/525 A and Mz120/478/S525A in the absence (free) or presence (bound) of argatroban (100 μM). Populations were fit to a double Gaussian distribution (red lines).
