Figure 1

Phenotype of B cell subpopulations in the bone marrow. B cell subtypes were subdivided in gates R2: B220^lowCD19⁻ cells, R3: B220^lowCD19⁺ cells, R4:- B220^highCD19^high, R5: B220^lowc-Kit^low cells, R6: B220^lowc-Kit⁻ cells, R7: B220^highc-Kit⁺ were quantified in Lgals3^+/+ (A and C) and Lgals3^−/− mice (B and D).Triple stained B220^lowCD19⁺c-Kit⁺ progenitor B cells (E) and B220^lowCD19⁺c-Kit⁻ B cells (F) were also measured in the bone marrow of Lgals3^+/+ (black bars) and Lgals3^−/− (white bars) mice. (G) Histograms show quadruple staining and quantity of B220⁺CD19⁺c-Kit⁺IL-7R⁺ B cells in the bone marrow of Lgals3^+/+ (gray histogram) and Lgals^−/− (white histogram) mice; Dashed histogram: Ig-isotype control. n = 5 mice per group. (*) Indicates p < 0.05. Dot plot graphs are representative of each mice group.