Figure 6 | Scientific Reports

Figure 6

From: Lack of galectin-3 modifies differentially Notch ligands in bone marrow and spleen stromal cells interfering with B cell differentiation

Figure 6

Notch target genes on B220+ cells sorted by FACS. (A) Notch-1 intracellular domain (NICD1) was significantly cleavage in Lgals3−/− B220+ cells and DLL4 was more evident in Lgals3+/+ B220+ cells. β-actin was used as endogenous control. (B) mRNA levels of DLL4 in B220+ cells of Lgals3+/+ and Lgals3−/− mice. (C) mRNA levels of JAG1 in B220+ cells of both mice groups. (D) mRNA levels of Notch target gene Hey-1 in B220+ cells of Lgals3+/+ and Lgals3−/− mice. n = 5 mice per group. (*) Indicates p < 0.05. (***) Indicates p < 0.01.

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