Figure 4
From: Specific Recognition of Arginine Methylated Histone Tails by JMJD5 and JMJD7
The specific binding between c-JMJD5/JMJD7 and bulk histone/synthetic peptides. A–L, are generated by Surface Plasmon Resonance Biacore, Y-axis, response RU, X-axis, time (seconds). M is generated by Fluorescence polarization, Y-axis, percentage of fluorescence intensity, X-axis, concentrations of peptides (μM). (A) c-JMJD5 specifically binds to bulk histone with significant binding affinity. (B) JMJD7 specifically binds to bulk histone with significant binding affinity. (C) c-JMJD5 binds to pH3R2me2a. (D) JMJD7 binds to pH3R2me2a. (E) c-JMJD5 binds to pH4R3me2a. (F) JMJD7 binds to pH3R4me2a. (G) c-JMJD5 binds to pH3R2(me2a)K4(me3). (H) JMJD7 fails to bind to pH3R2(me2a)K(me3). (I) c-JMJD5 fails to bind to pN-ac-H4R3(me2a)(N-terminal acetylated H4R3 peptide). (J) JMJD7 failed to bind to pN-ac-H4R3(me2a). (K) c-JMJD5 does not bind to pH4R3(me2a)K5(ac)K8(ac)K12(ac)K16(ac). (L) JMJD7 binds to pH4R3(me2a)K5(ac)K8(ac)K12(ac)K16(ac). Note: pH3R2 = pH3R2me2a, pH4R3 = pH4R3me2a, pH3R2K4 = pH3R2(me2a)K4(me3), pN-ac-H4R3 = pN-ac-H4R3(me2a), pH4R3 + AC = pH4R3(me2a)K5(ac)K8(ac)K12(ac)K16(ac), pH3R2 + AC = pH3R2(me2a)K4(ac)K9(ac)K14(ac)K18(ac).
