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Figure 1

From: Distinguishing Human Peripheral Blood NK Cells from CD56dimCD16dimCD69+CD103+ Resident Nasal Mucosal Lavage Fluid Cells

Figure 1

Isolation and phenotyping of NLF CD56+ cells. (A) Flow chart of experimental methods to validate CD56+ NK/ILC1 enrichment (B) Bulk NLF cell scatter properties, and stained for CD45, CD16, and CD56, (C) Diff-quick stain of adherent cells post- CD56+ cell enrichment procedure, demonstrating that the remaining adherent cells are a mixture of epithelial cells, neutrophils, and other non-NK cells, leaving an enriched CD56+/NK cell population in the supernatant. (D) CD56/CD16 phenotyping of post-enrichment NLF cell samples: Enriched NLF NK/ILCs (blue), CD16 compensation control (yellow), CD56 compensation control (red). All plots/images are representative of n = 3 individuals. (E) Median cell counts and viability pre- and post-enrichment, n = 18 individuals. Mean ± SD.

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