Figure 4

Binding of CHC in mouse kidneys during cold storage. Mouse kidneys were perfused with CHC-FITC, and fractions of the venous flow-through were collected and the fluorescence was measured (A; red line). The kidneys were then stored at 4 °C for 5 h, and subsequently washed with saline collected in fractions from the renal vein (A; black line). The kidneys were then fixed, sectioned and stained with CD31 (red) in order to detect the vasculature and investigate the distribution of CHC-FITC (green) in mouse kidneys. Kidneys treated with saline alone (B) were used as a background control for the FITC signal. Kidneys treated with CHC-FITC (C; left- and rightmost panels) had a clear signal in the vicinity of the vasculature (C; middle and right panels). Increased magnification showed CHC-FITC in the kidney vasculature (D) with some variability in the amount of FITC between different glomeruli. Images are representative of three separate experiments, and scale bars = 1 mm.