Figure 6
JNK1−/− but not WT Tregs inhibit IL-17 and IL-21-mediated inflammatory responses (a) Liver cells from C57BL/6 mice were isolated and cultured with BALB/c mouse pancreatic islets at a ratio of 10000:1 (1 × 105 liver cells and 10 islets) in the presence or absence of Tregs from WT C57BL/6 or JNK1−/− (1 × 104) mice. After 72 hours, various cytokine levels were measured by multiplex ELISA. Data from three independent experiments are shown. Mean values, p values and SEs are shown (b). Pancreatic islets and Tregs from WT or JNK−/− mice were transferred to CDM as described in Fig. 1. Fifteen and thirty days after transplantation, liver cell homogenates were prepared, and various cytokine levels were measured by multiplex ELISA. Data from five independent experiments are shown. Five mice per group were used. Mean values, p values and SEs are shown.
