Figure 5
From: Isolation of uracil auxotroph mutants of coral symbiont alga for symbiosis studies
Symbiosis experiments using wild type and T01 cells. (A) Stereomicrographs using bright field (Anemone) or a green excitation filter set (Algae: chlorophyll autofluorescence of Symbiodinium) photographed with time intervals shown above. Representative images of individuals are shown for each treatment. (B) Effect of uracil on the symbiotic state of T01. Symbiodinium area ratios were quantified by comparing signal regions of chlorophyll and normalized to the value of day 0 in the presence (closed bar) or absence (open bar) of uracil (*p < 0.05, n = 3, t-test). (C) Effects of uracil on the cell growth rate of WT and T01 in ASW liquid culture. WT (square) and T01 (circle) cells were grown in the presence (closed symbol) or absence (open symbol) of uracil (*adjusted p for interaction <0.05, n = 3, t-test).
