Figure 1 | Scientific Reports

Figure 1

From: Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand

Figure 1

PCR stop assay. G4-forming regions [c-MYC (191 bp), c-KIT (192 bp), BCL2 (398 bp) and VEGFA (192 bp)] and non-G4-forming regions [MBD3L3 (213 bp), CD4 (222 bp), CNDP2 (194 bp), and SOD1 (207 bp)] were amplified from 100 ng of HeLa genomic DNA by PCR in the presence of 1000 nM (lane 1), 100 nM (lane 2), 10 nM (lane 3), 1.0 nM (lane 4) or the absence of 7OTD (lane 5). As a control, PCR was performed without template DNA (lane 6). Lane M contains DNA markers. PCR products were analysed on 2% agarose gel. The cropped gels are used in the figure, and full-length gels are shown in Fig. S1.

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