Figure 5
TRAP ELISA responses. (A) Responses 1 week and 4 weeks post-MVA ME-TRAP responses for volunteers boosted at 4 weeks compared to 8 weeks post ChAd63 ME-TRAP. No significant difference at 1 or 4 weeks post boost, Mann Whitney test p = 0.3847 and 0.9754, respectively. (B) Responses 4 weeks post-MVA ME-TRAP response following single, double and triple ChAd63 ME-TRAP priming vaccination(s), with single MVA ME-TRAP boost. No significant difference between groups by Kruskal-Wallis ANOVA between all groups with Dunn’s correction p = 0.1597. (C) Response 4 weeks post final MVA ME-TRAP. No significant difference between groups by Kruskal-Wallis ANOVA between all groups with Dunn’s correction p = 0.4277. (D) Peak ELISA response following each ChAd63 ME-TRAP response per volunteer. Trend to higher responses after 2nd ChAd63 ME-TRAP vaccination compared to 1st ChAd63 ME-TRAP vaccination p = 0.0735 by Wilcoxon matched pairs test. Trend to higher responses after 1st ChAd63 ME-TRAP compared to 3rd ChAd63 ME-TRAP p = 0.0585. No significant difference between ChAd63 ME-TRAP 2 and ChAd63 ME-TRAP 3 p = 0.2932. (E) Peak ELISA response following each MVA ME-TRAP vaccination. Significantly higher response after 2nd MVA ME-TRAP compared to 1st MVA ME-TRAP by Wilcoxon matched pairs test between MVA ME-TRAP 1 and MVA ME-TRAP 2, p = 0.0103. No significant differences between MVA ME-TRAP 2 and MVA ME-TRAP 3 p = 1.000 or MVA ME-TRAP 1 and MVA ME-TRAP 3 p = 0.6250. (F) TRAP antibody titre 24 weeks post-last vaccination. No significant difference between groups by Kruskal-Wallis ANOVA with Dunn’s correction p = 0.466. (G) Individual AUC response for day 0 to 4 weeks post 1st MVA ME-TRAP vaccination. No significant difference between groups by Kruskal-Wallis ANOVA with Dunn’s correction p = 0.7932, (H) Individual AUC response for day 0 to 4 weeks post final MVA ME-TRAP vaccination. No significant difference between groups by Kruskal-Wallis ANOVA with Dunn’s correction between all groups, p = 0.0712. Median with IQR displayed.
