Table 4 Mean Normalised Gene Expression Levels in Aorta.

From: The influence of a high fat diet on bone and soft tissue formation in Matrix Gla Protein knockout mice

Sex	Males				Females
Genotype	WT		KO		WT		KO
Genotype	Mean (SD)		Mean (SD)		Mean (SD)		Mean (SD)
Diet	Control	High Fat	Control	High Fat	Control	High Fat	Control	High Fat
Diet	(n = 8)	(n = 14)	(n = 8)	(n = 7)	(n = 11)	(n = 11)	(n = 9)	(n = 6)
Osteocalcin	0.00005 (0.00001)^a	0.00004 (0.00001)^ab	0.00002 (0.00001)^b	0.00002 (0.00001)^ab	0.00004 (0.00004)	0.00002 (0.00001)	0.00004 (0.00006)	0.00006 (0.00003)
Gas6	0.009 (0.003)	0.014 (0.005)	0.014 (0.006)	0.013 (0.006)	0.010 (0.003)^a	0.014 (0.007)^ab	0.013 (0.005)^ab	0.018 (0.006)^b
Periostin	0.10 (0.02)	0.09 (0.03)	0.08 (0.05)	0.09 (0.04)	0.08 (0.02)	0.09 (0.04)	0.07 (0.03)	0.10 (0.04)
Mgp	0.013 (0.004)^a	0.016 (0.008)^a	0.001 (0.000)^b	0.001 (0.001)^b	0.017 (0.009)^a	0.016 (0.008)^a	0.001 (0.001)^b	0.001 (0.001)^b
Ggcx	0.0006 (0.0003)^a	0.0005 (0.0002)^ab	0.0003 (0.0001)^b	0.0004 (0.0001)^ab	0.0006 (0.0002)^a	0.0005 (0.0002)^a	0.0002 (0.0001)^b	0.0004 (0.0002)^ab
Vkor	0.005 (0.002)	0.005 (0.001)	0.003 (0.001)	0.003 (0.001)	0.005 (0.002)	0.005 (0.002)	0.003 (0.001)	0.004 (0.001)
Bmp-1	0.011 (0.004)^ab	0.013 (0.006)^a	0.007 (0.003)^b	0.007 (0.002)^ab	0.009 (0.001)^ab	0.012 (0.007)^a	0.006 (0.003)^b	0.009 (0.001)^ab
Bmp-2	0.0001 (0.0000)	0.0002 (0.0001)	0.0001 (0.0000)	0.0001 (0.0001)	0.0001 (0.0001)	0.0001 (0.0001)	0.0001 (0.0001)	0.0001 (0.0001)
Bmp-4	0.014 (0.007)^a	0.014 (0.002)^a	0.005 (0.002)^b	0.004 (0.002)^b	0.013 (0.005)^a	0.011 (0.005)^ab	0.007 (0.004)^ab	0.005 (0.003)^b
Leptin	0.003 (0.002)^a	0.006 (0.004)^ab	0.010 (0.005)^bc	0.014 (0.003)^c	0.005 (0.004)	0.007 (0.006)	0.008 (0.003)	0.010 (0.008)
Leptin Receptor	0.002 (0.001)	0.003 (0.002)	0.002 (0.001)	0.005 (0.006)	0.002 (0.001)	0.003 (0.001)	0.003 (0.002)	0.006 (0.007)
Sclerostin	0.011 (0.005)^a	0.017 (0.004)^b	0.002 (0.002)^c	0.003 (0.002)^c	0.009 (0.005)^ab	0.011 (0.005)^b	0.003 (0.002)^c	0.004 (0.005)^ac
Estradiol	ND	ND	ND	ND	ND	ND	ND	ND
Estradiol Receptor α	0.008 (0.003)^a	0.007 (0.003)^ab	0.004 (0.002)^b	0.007 (0.002)^ab	0.008 (0.002)^ab	0.010 (0.003)^b	0.004 (0.002)^c	0.006 (0.003)^ac
Estradiol Receptor β	ND	ND	ND	ND	ND	ND	ND	ND
ACE	0.02 (0.01)	0.03 (0.01)	0.03 (0.02)	0.03 (0.01)	0.02 (0.01)^a	0.03 (0.02)^a	0.04 (0.01)^b	0.02 (0.01)^a
Angiotensinogen	0.002 (0.003)^a	0.001 (0.000)^a	0.008 (0.005)^b	0.007 (0.004)^ab	0.004 (0.003)^a	0.001 (0.000)^a	0.010 (0.003)^b	0.004 (0.002)^a
Vegf ₁₂₀	0.005 (0.005)^a	0.011 (0.006)^ab	0.012 (0.004)^ab	0.017 (0.003)^b	0.007 (0.004)^a	0.010 (0.003)^ab	0.014 (0.004)^b	0.012 (0.004)^ab
Vegf ₁₆₄	0.022 (0.010)	0.028 (0.014)	0.024 (0.014)	0.038 (0.004)	0.016 (0.005)	0.028 (0.008)	0.025 (0.012)	0.023 (0.012)
Vegf ₁₈₈	0.048 (0.017)^a	0.038 (0.021)^ab	0.019 (0.007)^b	0.048 (0.012)^a	0.028 (0.010)	0.033 (0.011)	0.024 (0.017)	0.040 (0.020)
Vegf _total	0.08 (0.03)	0.08 (0.04)	0.06 (0.03)	0.11 (0.02)	0.06 (0.02)	0.07 (0.02)	0.07 (0.03)	0.08 (0.03)
Gapdh	2.5 (1.0)^a	1.4 (0.7)^a	0.3 (0.2)^b	0.6 (0.6)^b	1.7 (0.5)^a	1.3 (0.5)^a	0.3 (0.2)^b	0.6 (0.2)^b

For each parameter measured, values with different superscript letters are significantly different from each other within the same sex (p < 0.05). ND – not detected.

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Table 4 Mean Normalised Gene Expression Levels in Aorta.

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