Figure 6
Polarized light images reveal a distinct difference in collagen maturity and fiber thickness between the Fibrin only and 0.7 mg/ml COL rings. (a) Rings with fibrin only displayed a mix of green, yellow and a few red fibers indicating a mixed composition of mostly immature collagen and some mature fibers. (b) Collagen in 0.7 mg/ml COL rings appeared as mature red fibers. The yellow and green colors signify the presence of thin fibers, and red indicates thick fibers. L = lumen side. Scale bars = 100 µm. (c) Collagen fiber type/maturity were measured using the polarized light images through green, yellow and red fiber quantification. Fibrin only rings exhibited more green fibers indicating less maturity, whereas 0.7 mg/ml COL rings displayed more red fibers indicating more maturity with a statistically significant difference (p ≤ 0.05).
