Figure 1 | Scientific Reports

Figure 1

From: True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template

Figure 1

Outline of the experimental approach. Plants were transformed with the LSL-PPO repair template (kmR) and the Cas9-PPO nuclease (pptR) (P1-P8) or with Cas9-PPO and LSL-PPO on one T-DNA (kmR) (PL1-PL3). P2, P3, P4, P5 and P8 plants were crossed with plants transformed with 35S-REP (hygR). Progeny was selected on kanamycin, phosphinothricin and hygromycin for the presence of all three T-DNA constructs (P2Rep, P3Rep, P4Rep, P5Rep and P8Rep). Seed from P3, P8, PL1-PL3 (Cas9-PPO and LSL-PPO), P2Rep, P3Rep, P4Rep, P5Rep, P8Rep (Cas9-PPO, LSL-PPO and Rep), were germinated on butafenacil resulting in 8 GT plants (highlighted in yellow).

Back to article page