Figure 5
From: Upregulation of SOX2-activated lncRNA ANRIL promotes nasopharyngeal carcinoma cell growth
ANRIL/β-catenin is essential for SOX2-mediated nasopharyngeal carcinoma progression. (A) Levels of SOX2 and β-catenin protein expression in HNE-1 and CNE2 after stably sh-SOX2 and shControl. (B) β-catenin protein expression analysis in HNE-1 and CNE2 with SOX2 shRNAs alone or in combination with ANRIL vector. (C) Schematic diagram indicated the β-catenin binding to ANRIL region by RNA IP assays. (D) TOP-FOP flash assay was performed to evaluate the effect of shANRIL on WNT/β-catenin pathway. (E) Correlation between ANRIL and β-catenin transcript determined by quantitative real-time RT-PCR in 20 human nasopharyngeal carcinoma specimens.
