Figure 3
Immune microenvironment time-course analysis following single-dose RFT. (a) 4T1 tumor volume in Balb/c mice following single RFT (41 °C, 30 mins; day of treatment indicated by black arrow) which were terminated for microenvironment analysis at either 24hrs (left), 48hrs (middle), or 120hrs (right) post-RFT (n = 5/group). (b) Representative flow cytometry scatter plots among CD45+ cells showing the increase in CD4+ and CD8+ T cell populations in tumor between a control and RF-treated mice 24 hours post-RFT. (c) Cumulative plots of CD4+ T cell percentages (top row) and CD8+ T cell percentages (bottom row) showing changes in T cell percentages among total viable cells 24hrs post-treatment for tumor (left column) and changes 24, 48, and 120hrs post-treatment in tumor-draining lymph node (right column). (n = 3–10/group). (d) Control vs. RF-treated tumor percentage of MDSC (CD11b+/Gr1+) 120hrs post-treatment with representative flow plots (left) and cumulative plots showing percentages among total viable cells (right) (n = 5–10/group). See Supplemental Figs S4 and S5 for flow cytometry gating strategy. Each point represents an individual mouse percentage and all percentages are out of total viable cells analzyed. Error bars represent SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
