Figure 1
From: Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
Identification of non-covalently cell wall-bound S. aureus proteins. (A) Schematic representation of the experimental set-up for identification of non-covalently cell wall-bound proteins. S. aureus cells were first separated from the growth medium by centrifugation (spin). Pelleted S. aureus cells were treated with KSCN to release the non-covalently cell wall-bound proteins. KSCN-extracted proteins were re-bound to cell wall fragments (CWF) and, subsequently, released again by KSCN incubation. Upon centrifugation, the resulting pellet and supernatant fractions were analyzed by LDS-PAGE (B). Upon Simply Blue safe staining of the gel, protein bands were excised and identified by MS as indicated.
