Table 1 Strains and plasmids used in this study.
From: Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins
Strain or plasmid | Relevant phenotype(s) or genotype(s) | Source or reference |
|---|---|---|
Strains | ||
L. lactis PA1001 | MG1363 pepN::nisRK, allows nisin-inducible expression, ΔacmA ΔhtrA | |
E. coli MC1061 | araD139 Δ(araA-leu)7697 ΔlacX74 galK16 galE15(GalS) λ- e14- mcrA0 relA1 rpsL150(strR) spoT1 mcrB1 hsdR2 | (Novagen, Madison, Wis) |
S. aureus NCTC 8325 | Propagating strain for typing phage 47 | |
S. aureus Newman | NCTC 8178 clinical isolate | |
S. aureus Newman Δspa Δsbi | S. aureus Newman spa sbi mutant | |
Plasmids | ||
pRE-USPnlic | ampR; camR; pRExLIC fused with E. coli pBR322 replicon | |
pERL | eryR; pERL fused with pSH71 replicon | |
pNG4210 | camR pNG400 derivative, containing BamHI/EcoRI-XbaI/NotI cloning sites, his6 followed by a Stop codon | |
pNZ:LIC:efb | Fusion of pRE-USPnlic with pERL containing efb (SAOUHSC_01114, aa 30–165) | This study |
pNZ:LIC:eap | Fusion of pRE-USPnlic with pERL containing eap (SAOUHSC_02161, aa 31–584) | This study |
pNZ:LIC:sle1 | Fusion of pRE-USPnlic with pERL containing sle1 (SAOUHSC_00427, aa 26–334) | This study |
pNZ:LIC:sa0710 | Fusion of pRE-USPnlic with pERL containing sa0710 (SAOUHSC_00773, aa 25–279) | This study |
pNZ:LIC:atl1 | Fusion of pRE-USPnlic with pERL containing atl1 (SAOUHSC_00994, aa 199–775) | This study |
pNZ:LIC:atl2 | Fusion of pRE-USPnlic with pERL containing atl2 (SAOUHSC_00994, aa 776–1256) | This study |
pNG4210:lukG | pNG4210 containing lukG with C-terminal his6 (SAOUHSC_02241, aa 30–338) | This study |
pNG4210:lukH | pNG4210 containing lukH with C-terminal his6 (SAOUHSC_02243, aa 33–351) | This study |
pNG4210:ssaA2 | pNG4210 containing ssaA2 with C-terminal his6 (SAOUHSC_02571, aa 28–267) | This study |
pNG4210:emp | pNG4210 containing emp with C-terminal his6 (SAOUHSC_00816, aa 27–340) | This study |
pNG4210:ftsL | pNG4210 containing ftsL with C-terminal his6 (USA300HOU_1120, aa 66–133) | |
