Table 3 Identified non covalently cell surface-bound proteins.

From: Human antibody responses against non-covalently cell wall-bound Staphylococcus aureus proteins

Uniprot ID	Gene names	AA	S ^e	Protein name
Proteins with known conserved non covalently cell wall binding domains
LysM (PS51782) ^a
A6QH29	NWMN_1389 ebpS	486		Elastin-binding protein EbpS
A0A0H3K686	NWMN_0055 spa	520	S	Immunoglobulin G binding protein A
A0A0H3K6S5	NWMN_0724 SA0710^c	279	S	Uncharacterized protein
A0A0H3K7F5	NWMN_0634	265	S	Secretory antigen SsaA-like protein
A0A0H3KAZ4	NWMN_0429 sle1^d	334	S	N-acetylmuramoyl-L-alanine amidase AAA
A0A0H3KG37	NWMN_1157 lytN	383	S	Cell-wall hydrolase LytN
GW (PS51780) ^a
A0A0H3K7X7	NWMN_0922 atl	1256	S	Bifunctional autolysin
SH3B (PS51781) ^a
A0A0H3K875	NWMN_1039	481		Phage amidase [Bacteriophage phiNM2]
A0A0H3K8J7	NWMN_1534	291	S	Probable cell wall amidase LytH
G5 (PS51109) ^a
A0A0H3KAI3	NWMN_2392	1501	S	Uncharacterized protein
Identified by surfacome profiling
A0A0H3K7X7	NWMN_0922 atl	1256	S	Bifunctional autolysin
A6QIG7	NWMN_1877 chp	149	S	Chemotaxis inhibitory protein precursor
A0A0H3KA75	NWMN_0166 coa	636	S	Coagulase
A6QF98	NWMN_0758 emp^d	340	S	Extracellular matrix protein-binding protein emp precursor
A0A0H3KF27	NWMN_2399 fnbA	741	S	Fibronectin binding protein A
A0A0H3K6R0	NWMN_0687	646	S	Lipoteichoic acid synthase
A6QK59	NWMN_2469 isaA	233	S	Probable transglycosylase IsaA precursor
A6QJQ7	NWMN_2317 sbi	436	S	Immunoglobulin-binding protein sbi precursor
A0A0H3KCA1	NWMN_1066	109	S	Uncharacterized protein Ehp
A0A0H3K6X4	NWMN_0362	203	S	Uncharacterized protein
A0A0H3KET4	NWMN_0585	168	S	Uncharacterized protein
A0A0H3K7N7	NWMN_0757	508	S	Secreted von Willebrand factor-binding protein
A0A0H3KEG7	NWMN_2199 ssaA2^d	267	S	Secretory antigen SsaA
LDS-PAGE: Gel band MS
1^b A6QIG2	NWMN_1872 eap^d	584	S	65 kDa membrane protein precursor
A6QDC3	NWMN_0083 deoB	392		Phosphopentomutase
2 A6QG68	NWMN_1078 argF	333		Ornithine carbamoyltransferase
3 A6QIG2	NWMN_1872 eap^d	584	S	65 kDa membrane protein precursor
4 A6QJQ7	NWMN_2317 sbi	436	S	Immunoglobulin-binding protein Sbi precursor
5 A0A0H3KHT5	NWMN_1928 lukH^d	351	S	Leukocidin/hemolysin toxin family S subunit
6A6QF98	NWMN_0758 emp^d	340	S	Extracellular matrix protein-binding protein Emp precursor
A0A0H3K9N1	NWMN_1927 lukG^d	338	S	Leukocidin/hemolysin toxin family F subunit
7A0A0H3K5Z1	NWMN_0249 hel^d	296	S	5′-nucleotidase, lipoprotein e(P4) family protein
A0A0H3KIY0	NWMN_2444	85		Uncharacterized protein
8A6QG59	NWMN_1069 efb^d	165	S	Fibrinogen-binding protein precursor

^aPROSITE ID of Motif; ^bExtracted band number from LDS-PAGE; ^cGene locus of NWMN_0724 homolog in S. aureus N315, used instead of gene name; ^dGene name of homologous protein if none was found in the Uniprot record; S^e Secretion signal predicted by SignalP and/or according to Uniprot.

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Table 3 Identified non covalently cell surface-bound proteins.

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