Table 1 Kinetic rate constants for FPR binding to prothrombin and its products.

From: Interplay between conformational selection and zymogen activation

 

k12 (s−1)

k21 (s−1)

k on (μM−1s−1)

k off (s−1)

Kd,app (μM)a

Kd,app (μM)b

E*:E

Prothrombin

6.9 ± 0.3

≥38 ± 3

≥1.5 ± 0.1

≥45 ± 5

≥200 ± 20

190 ± 20

≥6:1

Prethrombin-1

42 ± 2

≥96 ± 8c

≥0.46 ± 0.05

42 ± 2

≥92 ± 8c

92 ± 8

≥2:1c

Prethrombin-2

170 ± 10

≥100 ± 10c

≥0.93 ± 0.08

27 ± 2

≥29 ± 2c

29 ± 2

≥1:2c

Meizothrombin

8.8 ± 0.5

3.4 ± 0.7

2.5 ± 0.3

0.8 ± 0.1

0.44 ± 0.03

0.51 ± 0.04

1:3

Thrombin

16 ± 1

3.8 ± 0.8

2.6 ± 0.3

0.6 ± 0.1

0.29 ± 0.02

0.28 ± 0.02

1:4

  1. aCalculated from eq. 3. bDetermined by direct fluorescence titration. cCalculated under the assumption that k on = 1.5 μM−1 s−1. Experimental conditions are: 400 mM ChCl, 50 mM Tris, 0.1% PEG8000, pH 8.0, at 15 °C.
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