Figure 2
From: Exogenous hydrogen sulfide gas does not induce hypothermia in normoxic mice
Schematic illustration of the experimental setup and design. The animals were allocated to one of the following experimental groups: (A) 80 ppm H2S in 21% O2 and 79% N2 (H2S in 21% O2 group, N = 12); (B) 80 ppm H2S in 17% O2 and 83% N2 (H2S in 17% O2 group, N = 6); (C) 5% O2 and 95% N2 (5% O2 group, N = 12); (D) 17% O2 and 83% N2 (17% O2 group, N = 6); and (E) 21% O2 and 79% N2 (normoxia group, N = 12). The experiments were performed at a mean ± SD Ta of 21.2 ± 0.6 °C, measured with a thermistor. During the whole experiment the mice were solitarily housed in a custom-built airtight cage and recorded with a thermographic camera (CAM) for 10 min every hour (red markers) for skin temperature- and locomotor activity analysis. After 1 h of baseline 21:79% O2:N2 exposure, each mouse was exposed to a gas mixture (A–E) for 6 h that was passed through the airtight cage, after which 6 of the animals in each group were allowed to recover at 21:79% O2:N2 for 3 h. The other 6 animals of group (A,C and E) were sacrificed for another study.
