Figure 4

Endosomal co-localization of the GLP-1/GLP-1R complex in alpha cells in non-diabetic and diabetic rats. Figure 4a was taken from the red rectangular area in Figs 1a and 4b is a merged image of the red GLP-1R and blue EEA-1. Figure 4c was taken from the red rectangular area in Figs 1b and 4d is a merged image of the red GLP-1R and blue EEA-1. Figure 4e was taken from the yellow rectangular area in Fig. 2b with red GLP-1R, green GLP-1 and blue Rab11, and Fig. 4f is a merged image of the red GLP-1R and blue Rab11 in Fig. 4e. The yellow arrows indicate red GLP-1 R, which is not located in alpha cells. The white arrows in Fig. 4c and e indicate the co-localization of GLP-1R and endosome markers EEA-1 and Rab11, respectively, in alpha cells.