Figure 4
From: Visualisation of DCP, a nerve agent mimic, in Catfish brain by a simple chemosensor
Representative photomicrographs of Catfish brain (ventral side up) showing cranial and caudal ends, hypothalamus, pituitary and optic chiasma during dissection (A) and after harvesting in chilled PBS (B). Brain slices from telencephalon (marked as diagonal rectangles in (B) were pre-incubated with ARC, washed with PBS, treated with the analyte DCP, washed again to remove excess DCP and observed under fluorescence inverted microscope at 5 min intervals (D,E from upper slice and G,H from lower slice). Parallel slices treated with ARC only served as negative control (C,F). Figures are representative of three independent observations from separate donors, scale bar ~1 mm (A,B); ~200 µm from (C to G) and ~400 µm for (H).
