Figure 3
From: Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice
Chloride-dependent alterations of Purkinje cell activity pattern. (A) Optical identified PCs in lobes III-V were used for whole-cell patch clamp recordings. Recording electrode attached to PC is depicted by white lines. (B) Using the current clamp mode, three different activity patterns of PCs were found: tonic firing, burst firing and inactive. (C) No differences in pattern distribution between NPC1+/+ and NPC1−/− mice were observed using a low Cl-[i] (NPC1+/+: N = 11, n = 29; NPC1−/−: N = 8, n = 30). (D) Significantly more tonic firing PCs were found in NPC1−/− mice using a high Cl−[i], whereas more PCs in NPC1+/+ mice showed burst activity or were inactive (NPC1+/+: N = 19, n = 52; NPC1−/−: N = 15, n = 43). (E) The tonic firing PCs of NPC1−/− mice showed a significantly reduced action potential frequency and (F) a higher spike regularity using a high Cl-[i] (NPC1+/+: N = 12, n = 14; NPC1−/−: N = 11, n = 25). *p < 0.05, **p < 0.01, data shown as mean ± sem. ML = molecular layer, PL = Purkinje layer, GL = granular layer. Barnard’s 2 × 2 test was used to determine significance for data shown in C and D. Student´s unpaired t-test was used to determine significance for data shown in E and F.
