Figure 5
From: A rice class-XIV kinesin enters the nucleus in response to cold
Dual localisation of full-length OsDLK-GFP in different plant models. (a) Dual localisation of full-length OsDLK-GFP in interphase cells of the rice leaf sheath. Nuclear and cortical OsDLK-GFP signals are indicated by red and yellow arrows, respectively. Scale bar: 20 µm. (b) Co-localisation of OsDLK-GFP and microtubules in Arabidopsis thaliana protoplasts. Representative maximum intensity projections of fluorescent image stacks showing transient expression of OsDLK-GFP (cyan), stable expression of the microtubule marker TuA-RFP (red), and chloroplast autofluorescence (blue) in an Arabidopsis thaliana mesophyll protoplast. Scale bar: 5 µm. (c) Representative maximum intensity projection of fluorescent image stacks showing transient expression of OsDLK-GFP (cyan) in Nicotiana benthamiana leaf pavement cells. Red arrows show the OsDLK-GFP localisation in the nuclei. Cell borders are indicated by red lines generated from bright field images taken at the same field of view. Scale bar: 25 µm.
