Figure 8
From: A rice class-XIV kinesin enters the nucleus in response to cold
OsDLK-GFP enters the nucleus in response to cold stress. (a) Progressive nuclear import of OsDLK-GFP with increasing time of cold treatment. Confocal sections collected from the cortical and nuclear planes are shown either for the GFP signal alone or merged with the differential interference contrast image to show the topology. CK represent cells cultivated at 25 °C serving as negative control. (b) Representative cells challenged by cold for 7 hours and triple stained for microtubules (immunofluorescence using a TRITC conjugated antibody), OsDLK-GFP, a merge of both signals, and DNA visualised by Höchst 33258. The white square has been magnified to show details of colocalisation (arrow). Scale bars: 10 μm. (c) Time course of non-transformed BY-2 cells mortality after treatment with ice water.
